Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RB1

Cell type

Cell type Class
Prostate
Cell type
C4-2
Tissue
Prostate
Cell Type
Epithelial
Disease
Prostate Cancer

Attributes by original data submitter

Sample

source_name
C4-2 cell
cell type
prostate cancer cell line
medium condition
In RPMI 1640 medium with 5% charcoal-stripped FBS (CSS)
cell line
C4-2
treatment
10nM dihydrotestosterone (DHT) for 4 hours
chip antibody
anti-RB (Cell Signaling)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were constructed using the SMARTer ThruPLEX DNA-Seq Prep Kit (Takara Bio USA).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
8530276
Reads aligned (%)
89.6
Duplicates removed (%)
3.6
Number of peaks
277 (qval < 1E-05)

hg19

Number of total reads
8530276
Reads aligned (%)
89.0
Duplicates removed (%)
4.5
Number of peaks
353 (qval < 1E-05)

Base call quality data from DBCLS SRA